Western Blot:Article Title: Etamsylate introduces platelets into priming state and enhances platelet procoagulant response - Functional and mechanistic update of a classical hemostatic agent.
Article Snippet: PPACK (D-Phe-pro-arg-chloromethyl ketone) was from Santa Cruz Biotechnology. .. DiOC6 (3-octadecyl-2-[3-(3-octadecyl-2 (3 H)-benzoxazolylidene)-1-propenyl]-,perchlorate), AlexaFluor647labeled annexin V, AlexaFluor488-labeled mouse monoclonal antihuman CD63 (Cat #: MA5–18149), Fura-2-AM, and DAPI (4′,6-diamidino-2-phenylindole), RestoreTM Western Blot Stripping Buffer, goat polyclonal anti-rabbit IgG antibody (HRP conjugate, Cat #: 31460), rabbit polyclonal anti-phospho-PLC gamma 2 (Tyr759) antibody (Cat #: BS-3339R), Halt© proteases and phosphatases inhibitor cocktail, Super SignalTM West Femto Substrate for chemiluminescence, and SpectraTM Multicolor Broad Range Protein Ladder (10–260 kDa, Cat#: 22634) were purchased from Thermo Fisher Scientific. .. FITC-mouse monoclonal anti-platelet antibody, clone PAC- (FITC-PAC-1, Cat #: 340507), FITCmouse monoclonal anti-human CD62P (P-selectin, Cat #: 555523), PEmouse monoclonal anti-human CD15b (Cat #: 567103, and its isotype control – Cat #: 554680), FITC-mouse monoclonal anti-human CD41a (Cat #: 555466, and its isotype control – Cat #: 555748), and PE-mouse monoclonal anti-human CD41a (Cat #: 557297) were from Beckton Dickinson.
Stripping:Article Title: Etamsylate introduces platelets into priming state and enhances platelet procoagulant response - Functional and mechanistic update of a classical hemostatic agent.
Article Snippet: PPACK (D-Phe-pro-arg-chloromethyl ketone) was from Santa Cruz Biotechnology. .. DiOC6 (3-octadecyl-2-[3-(3-octadecyl-2 (3 H)-benzoxazolylidene)-1-propenyl]-,perchlorate), AlexaFluor647labeled annexin V, AlexaFluor488-labeled mouse monoclonal antihuman CD63 (Cat #: MA5–18149), Fura-2-AM, and DAPI (4′,6-diamidino-2-phenylindole), RestoreTM Western Blot Stripping Buffer, goat polyclonal anti-rabbit IgG antibody (HRP conjugate, Cat #: 31460), rabbit polyclonal anti-phospho-PLC gamma 2 (Tyr759) antibody (Cat #: BS-3339R), Halt© proteases and phosphatases inhibitor cocktail, Super SignalTM West Femto Substrate for chemiluminescence, and SpectraTM Multicolor Broad Range Protein Ladder (10–260 kDa, Cat#: 22634) were purchased from Thermo Fisher Scientific. .. FITC-mouse monoclonal anti-platelet antibody, clone PAC- (FITC-PAC-1, Cat #: 340507), FITCmouse monoclonal anti-human CD62P (P-selectin, Cat #: 555523), PEmouse monoclonal anti-human CD15b (Cat #: 567103, and its isotype control – Cat #: 554680), FITC-mouse monoclonal anti-human CD41a (Cat #: 555466, and its isotype control – Cat #: 555748), and PE-mouse monoclonal anti-human CD41a (Cat #: 557297) were from Beckton Dickinson.
Isolation:Article Title: CAR-T cells with the CD38 - CD73 - Tim-3 - HLA-DR + phenotype predict the efficacy of tisagenlecleucel as a treatment for B cell precursor ALL.
Article Snippet: Monitoring of tisa-cel transgene DNA using ddPCR DNA was purified from cryopreserved mononuclear cells isolated from BM aspirates or PB using QIAmp DNA blood mini kits (- QIAGEN). .. Isolated DNA was quantified by Qubit double-stranded DNA (dsDNA) broad-range assay kits (Thermo Fisher Scientific) and stored at − 30◦C. .. Primers and probes targeting the CDKN1A gene and the junction region of the 4-1BB co-stimulatory domain and the CD3-zeta signaling region of the tisa-cel transgene were designed as described previously.22 The TaqMan non-fluorescent quencher-minor groove binder (NFQ-MGB) probes (fluorescence: FAM for CDKN1A, VIC for CAR) were supplied by Thermo Fisher Scientific. ddPCR were performed using a QX200 AutoDG ddPCR system (Bio-Rad) and a C1000 Touch thermal cycler (Bio-Rad).
Concentration Assay:Article Title: Advances in Functional Foods: Using Double Emulsion Gels to Deliver CBD and Probiotics and to Modulate Human Gut Microbial Communities
Article Snippet: .. DNA concentration was recorded by the Qubit® 3.0 fluorometer using the double-stranded DNA (dsDNA) broad range (BR) assay kit (Invitrogen, Eugene, OR, USA). .. The composition of microbial community was analyzed by next-generation sequencing (NGS) of V2-4 and V6-9 regions using the Ion TorrentTM Next-Generation Sequencer (Thermo Fisher Scientific, Carlsbad, CA, USA).
Protease Inhibitor:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
Electroporation:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
Polymerase Chain Reaction:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
Bicinchoninic Acid Protein Assay:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
Real-time Polymerase Chain Reaction:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
Next-Generation Sequencing:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
Chromatin Immunoprecipitation:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
RNA Sequencing:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
Cloning:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
CRISPR:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
Recombinant:Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SbfI NEB R3642 T4 DNA ligase NEB M0202S DpnI NEB R0176S T4 polynucleotide kinase (PNK) NEB EK0031 NruI-HF NEB R3192S PmlI NEB R0532S Quick Calf Intestinal Phosphatase (CIP) NEB M0525S BamHI-HF NEB R3136S MluI-HF NEB R3198S BssHII NEB R0199S BbsI-HF NEB R3539S QuickExtract solution Lucigen QE0905T T7 Endonuclease 1 (T7E1) enzyme NEB M0689S TRIzol ThermoFisher 15596018 Turbo DNase Invitrogen AM2238 Power SYBR reaction mastermix ThermoFisher 4368706 Poly-L-lysine hydrobromide Merck/Sigma P2636 HaloTag ligand TMR Promega G8251 Hoechst 33342 trihydrochloride trihydrate Invitrogen by ThermoFisher H3570 Dulbecco’s Phosphate-buffered saline ThermoFisher 14190250 Gibco FluoriBrite ThermoFisher Scientific A1896701 Gibco 1M HEPES ThermoFisher Scientific 15630106 HaloTag Janelia Fluor 549 Promega GA1110 HaloTag Janelia Fluor 646 Promega GA1120 1,6 - Hexanediol Merck 240117 100X Halt protease and phosphatase inhibitor cocktail Fisher 10085973 RNase A NEB T3018 32% Paraformaldehyde solution EM Grade Electron Microscopy Sciences 15714-S Bovine Serum Albumin (BSA) Sigma-Aldrich A3059-100g VECTORshield that contained 4,6-diamidino-2phenylindole (DAPI) Vector laboratories H-2000-10 A-485 Selleckchem S8740 Trichostatin A (TSA) Stratech, APExBIO A8183 Dithiothreitol (DTT) Melford MB1015-25G Sodium butyrate Sigma-Aldrich 303410-100g Benzonase Nuclease Sigma-Aldrich E1014-25KU Trans-blot turbo mini 0.2 μm nitrocellulose transfer packs Biorad 1704158 IGEPAL (NP40) CA-630 Sigma 18896-50mL PMSF SLS 10837091001 acetyl Coenzyme A sodium salt solution Sigma A2056-10MG recombinant polynucleosomes H3.1 Active Motif 31466 NuPAGE 3–8% Tris-Acetate gel Invitrogen by thermofisher EA0378BOX Tris-Acetate SDS running buffer Novex by life technologies LA0041 NuPAGE 4–12% Bis-Tris gel Invitrogen by thermofisher NP0335BOX MOPS SDS running buffer (20X) Invitrogen by thermofisher NP0001 16% Formaldehyde Solution (w/v), Methanol-free ThermoFisher Scientific 28906 (Continued on next page) Cell Reports 45, 117109, March 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glycine Fisher 56-40-6 cOmplete TM , Mini, EDTA-free Protease Inhibitor Cocktail Merck 11836170001 Sodium deoxycholate Sigma-Aldrich D6750-100g N-lauroylsarcosine sodium salt Sigma L5777-50g Proteinase K NEB P8107G Dimethyl sulfoxide (DMSO) anhydrous, 10 × 3mL for resuspending dyes/ligands Invitrogen D12345 NuPAGE Sample Reducing Agent (10X) ThermoFisher NP0009 NuPAGE LDS Sample Buffer (4X) ThermoFisher NP0007 Critical commercial assays Neon electroporation system and 100 μL kit ThermoFisher MPK10096 Monarch PCR cleanup kit NEB T1030L Qubit dsDNA Broad Range BR kit ThermoFisher Q32853 Qubit dsDNA High Sensitivity (HS) kit ThermoFisher Q33230 Qubit RNA Broad Range (BR) Assay Kit ThermoFisher Q10210 High Capacity cDNA kit ThermoFisher 4387406 Pierce BCA Protein Assay Kit ThermoFisher 23227 NEBNext UltraTMII DNA Library Prep Kit for Illumina NEB E7645 TapeStation High Sensitivity D1000 ScreenTape Agilent 5067–5584 CUT&RUN Assay kit Cell Signaling 86652S Quant-seq 3 ′ mRNA-Seq Library Prep kit Lexogen 191.24 qPCR Add-on Kit Lexogen 208.96 ChromoTek GFP-Trap® Magnetic Particles M-270 Proteintech gtd-20 Protein G Dynabeads TM Fisher 10004D AMPure XP beads Beckman Coulter A63880 Deposited data The high-throughput sequencing data from this study have been deposited to the GEO database grouped under SuperSeries GSE299618. https://www.ncbi.nlm.nih.gov/geo/ GEO: GSE299618 ChIP-seq This study GEO: GSE268266 CUT&RUN This study GEO: GSE299613 RNA-seq This study GEO: GSE299614 Experimental models: Cell lines HEK293T ATCC CRL-3216 GP2-293 Clontech 631458 CBP-Halo HEK293T (clonal) This paper Available on request Doxycycline inducible CBP-WT-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR6-GFP HEK293T This paper Available on request Doxycycline inducible CBP-ΔIDR7-GFP HEK293T This paper Available on request Oligonucleotides Primers for cloning IDT See Table S1 Primers for qPCR IDT See Table S1 Synthetic DNA sequences IDT See Table S1 CRISPR gene editing IDT See Table S1 Recombinant DNA pRetroX-pTRE3G Clontech Cat#631530 pRetroX-Tet3G Clontech Cat#631530 (Continued on next page) 20 Cell Reports 45, 117109, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER pVSV-G Clontech Cat#631530 pRetroX-TRE3G_CBP-GFP This study Addgene #221926 pRetroX-TRE3G_CBPΔIDR6-GFP This study Addgene #221927 pRetroX-TRE3G_CBPΔIDR7-GFP This study Addgene #221928 pAcGFP-N1_CBP This study Addgene #221903 pAcGFP-N1_CBPdIDR1 This study Addgene #221904 pAcGFP-N1_CBPdIDR3 This study Addgene #221905 pAcGFP-N1_CBPdIDR4 This study Addgene #221906 pAcGFP-N1_CBPdIDR6 This study Addgene #221907 pAcGFP-N1_CBPdIDR7 This study Addgene #221908 pAcGFP-N1_CBPdCFID This study Addgene #221909 pAcGFP-N1_CBPdAL This study Addgene #221910 pAcGFP-N1_CBPKTG This study Addgene #221911 pAcGFP-N1_CBPdIDR6dAL This study Addgene #221912 pAcGFP-N1_CBPdIDR6KTG This study Addgene #221913 pAcGFP-N1_CBPdIDR7dAL This study Addgene #221914 pAcGFP-N1_CBPdIDR7KTG This study Addgene #221915 pAcGFP-N1_CBPdCFIDdAL This study Addgene #221916 pAcGFP-N1_CBPdCFIDKTG This study Addgene #221917 pHR-mCh-Cry2WT Gift from Clifford Brangwynne Addgene #101221 pHR-mCh-Cry2WT-NLS This study Addgene #221925 pHR-IDR1-mCh-Cry2WT-NLS This study Addgene #221918 pHR-IDR3-mCh-Cry2WT-NLS This study Addgene #221919 pHR-IDR4-mCh-Cry2WT-NLS This study Addgene #221920 pHR-AL-mCh-Cry2WT-NLS This study Addgene #221924 pHR-IDR6-mCh-Cry2WT-NLS This study Addgene #221921 pHR-IDR7-mCh-Cry2WT-NLS This study Addgene #221922 pHR-CFID-mCh-Cry2WT-NLS This study Addgene #221923 pAcGFP-N1_CBPΔIDR6H2-GFP This study Addgene #221929 pAcGFP-N1_CBPΔIDR7H1-GFP This study Addgene #221930 pAcGFP-N1_CBPIDR6-Shuffle-GFP This study Addgene #247245 pAcGFP-N1_CBPIDR7-Shuffle-GFP This study Addgene #247246 pSpCas9(BB)-2A-GFP (PX458) Gift from Feng Zhang Addgene plasmid # 48138 Software and algorithms UCSC CRISPR targets tool https://genome.ucsc.edu/ N/A Evaluation tool on the E-CRISP site https://e-crisp.org/ N/A Inference of CRISPR Edits (ICE) Synthego 57 ; https://ice.synthego.com/#/ N/A ImageJ/Fiji Schindelin et al. 58 N/A Stack Registration (StackReg) Thé venaz et al. 59 N/A Zen Blue software Zeiss N/A Zeiss Arivis Pro/Vision4D version 3.4 Zeiss N/A R in RStudio R Core Team 60,61 N/A GraphPad prism Prism (San Diego 2022) N/A FastQC v0.11.9 http://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ N/A Cutadapt v3.4 Martin 62 N/A Trim Galore https://www.bioinformatics.babraham.ac.uk/ projects/trim_galore/ N/A Burrows-Wheeler Alignment, v0.7.17-r1188 Li and Durbin 63 N/A Picard v2.27.4 https://broadinstitute.github.io/picard/ N/A (Continued on next page) Cell Reports 45, 117109, March 24, 2026 21
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